Expression andin vitrocleavage activity of anti-caspase-7 hammerhead ribozymes
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چکیده
منابع مشابه
Minimal Hammerhead Ribozymes with Uncompromised Catalytic Activity.
We report here that a single additional trans-Hoogsteen base-pairing interaction in the minimal hammerhead ribozyme transforms an RNA sequence possessing typically modest catalytic activity into one possessing greatly enhanced catalytic activity that is instead typical of full-length natural hammerhead RNAs that have additional extensive tertiary contact interactions. Formation of this addition...
متن کاملExperimental cancer gene therapy by multiple anti-survivin hammerhead ribozymes.
To improve the efficacy of gene therapy for cancer, we designed four hammerhead ribozyme adenoviruses (R1 to R4) targeting the exposed regions of survivin mRNA. In addition to the in vitro characterization, which included a determination of the sequence specificity of cleavage by primer extension, assays for cell proliferation and for in vivo tumor growth were used to score for ribozyme efficie...
متن کاملIntronic hammerhead ribozymes in mRNA biogenesis
Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes along the tree of life. In eukaryotes, most of the genomic HHRs se...
متن کاملIntronic hammerhead ribozymes in mRNA biogenesis
Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes along the tree of life. In eukaryotes, most of the genomic HHRs se...
متن کاملIntronic hammerhead ribozymes in mRNA biogenesis
Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes along the tree of life. In eukaryotes, most of the genomic HHRs se...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2004
ISSN: 1007-9327
DOI: 10.3748/wjg.v10.i14.2078